Vis-NIR spectroscopy has widely been used to study egg freshness, internal defects, fertility, hatching time and embryo growth, and is reliable for scientific application and industrial uses. Given the yolk has a higher absorbance coefficient than albumen over a wide range of the UV-Vis-NIR spectrum, and bigger yolks absorb more light energy compared to smaller ones, this provides potential noninvasive means of detecting small differences in the amount of yolk ratio from spectral differences during light transmission.